Composite

Part:BBa_K4905012:Design

Designed by: Merel van den Bosch   Group: iGEM23_TU-Eindhoven   (2023-08-15)


Elastin-Like Polypeptide Triblock with FKBP12 and FRB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2177
    Illegal EcoRI site found at 4103
    Illegal XbaI site found at 294
    Illegal XbaI site found at 2220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2177
    Illegal EcoRI site found at 4103
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2177
    Illegal EcoRI site found at 4103
    Illegal BglII site found at 225
    Illegal XhoI site found at 2194
    Illegal XhoI site found at 4120
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2177
    Illegal EcoRI site found at 4103
    Illegal XbaI site found at 294
    Illegal XbaI site found at 2220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2177
    Illegal EcoRI site found at 4103
    Illegal XbaI site found at 294
    Illegal XbaI site found at 2220
    Illegal NgoMIV site found at 351
    Illegal NgoMIV site found at 531
    Illegal NgoMIV site found at 621
    Illegal NgoMIV site found at 801
    Illegal NgoMIV site found at 2277
    Illegal NgoMIV site found at 2457
    Illegal NgoMIV site found at 2547
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ELPs are repetitive sequences that are not suitable for PCR. Colony PCR is needed to obtain more of the DNA.


Source

https://pubs.acs.org/doi/10.1021/ja043277y

References